Measurement

Part:BBa_J107252

Designed by: Lorenzo Pasotti, Massimo Bellato, Angelica Frusteri   Group: Magni Lab   (2021-05-27)


HSL-inducible cassette for sgRNA (optimized) targeting R0040-driven RFP

HSL-inducible cassette (optimized) for sgRNA targeting R0040 and Ptet-driven RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3010
    Illegal AgeI site found at 3122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2087
    Illegal BsaI site found at 2265
    Illegal BsaI.rc site found at 706
    Illegal BsaI.rc site found at 1839


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